Background: Luteinizing hormone (LH) plays a vital role in induced ovulation and controlled ovarian hyperstimulation in human assisted reproduction. This evaluation has a brief review of the roles of LH in supporting follicular development and function of LH Ovulation Rapid Test Cassette in preparing pregnancy. A rapid increase in LH plasma, called an LH surge, induces ovulation within 2-3 days. The surge can be detected by the observation of LH in serum or urine with ovulation predictor kits (OPKs). If a woman knows the duration of her menstrual cycle, she can plan the timing of testing. The average length of the menstrual cycle is 28 days, but a regular cycle lasting anywhere between 24 and 38 days is considered normal. The cycle length is determined by follicular growth and by the lifespan of the corpus luteum [1]. Many women experience varying cycle lengths with back and forth shifts in the day of ovulation, which may pose a problem especially for infertility patients. To detect ovulation, patients are required to determine LH levels in urine with an OPK daily until getting a positive result, which causes undue stress in addition to the financial burden.