Background: Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy.Glycosylation of hCG is known to be essential for its biological activity. “Hyperglycosylated” variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases. Therefore, the hCG Pregnancy Rapid Test Cassette (Urine) can detect the gestational diseases during early pregnancy which plays an important role in gynaecology. Human chorionic gonadotropin (hCG) is an essential pregnancy-associated glycoprotein that is a member of the same family that includes luteinizing hormone, follicle stimulating hormone and thyroid stimulating hormone. Each member is a heterodimer consisting of shared α-subunits in non-covalent association with distinct β-subunits that confer their specific physiological activities. Pregnancy associated hCG is mainly secreted by the syncytiotrophoblast (ST) associated with the embryo and is excreted in urine. hCG levels in maternal blood increase progressively during early pregnancy, reaching maximal levels between the 8-11th week of gestation. Thereafter, the level of hCG declines until around twenty weeks and then remains comparatively low for the remainder of the pregnancy. The functional roles of hCG throughout pregnancy have been extensively studied and are thought to be necessary for the maintenance of pregnancy by mediating multiple placental, uterine and fetal functions. hCG may play a part in implantation through its receptors on the endometrium and is thought to aid smooth muscle relaxation, maintain quiescence of the myometrium in pregnancy and promote myometrial vasodilatation.